Nanodisk-Associated Amphotericin B Clears Leishmania major Cutaneous Infection in Susceptible BALB/c Mice

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Nanodisk-associated amphotericin B clears Leishmania major cutaneous infection in susceptible BALB/c mice.

Nanometer-scale, apolipoprotein-stabilized phospholipid bilayer disk complexes (nanodisks [ND]) harboring the toxic and poorly soluble antileishmanial agent amphotericin B (AMB) were examined for efficacy in treatment of Leishmania major-infected BALB/c mice (Mus musculus). L. major-infected mice were intraperitoneally (i.p.) treated with AMB-ND in 0-, 1-, and 5-mg/kg doses at 24 h, 48 h, and 4...

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Effect of Amphotericin B Nanodisks on Leishmania major Infected Mice

OBJECTIVE To assess the efficacy of a novel formulation of the polyene antibiotic, amphotericin B (AMB), as therapy for cutaneous leishmaniasis in different mouse strains. METHODS (AMB), was formulated into water-soluble transport particles, termed nanodisks (ND). Balb/c and CH3 mice infected with Leishmania major on Day 0 were administered vehicle alone, empty ND or AMB-ND on Day 1 and day 7...

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Migration-inhibitory factor gene-deficient mice are susceptible to cutaneous Leishmania major infection.

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Protective and antifungal properties of Nanodisk-Amphotericin B over commercially available Amphotericin B☆

Objective Amphotericin B (AMB), a potent antifungal agent, has been employed as topical and systemic therapy for sinonasal fungal infections. A novel formulation of nanodisc (ND) containing super aggregated AMB (ND-AMB) for the treatment of fungal infections has been recently developed to provide greater protection from AMB toxicity than current, clinically approved lipid-based formulations. Th...

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Amphotericin B induces interdigitation of apolipoprotein stabilized nanodisk bilayers.

Amphotericin B nanodisks (AMB-ND) are ternary complexes of AMB, phospholipid and apolipoprotein organized as discrete nanometer scale disk-shaped bilayers. In gel filtration chromatography experiments, empty ND lacking AMB elute as a single population of particles with a molecular weight in the range of 200 kDa. AMB-ND formulated at a 4:1 phospholipid:AMB weight ratio separated into two peaks. ...

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ژورنال

عنوان ژورنال: Antimicrobial Agents and Chemotherapy

سال: 2006

ISSN: 0066-4804,1098-6596

DOI: 10.1128/aac.50.4.1238-1244.2006